Comparative Study of Marketed and Novel Colloidal Formulation for Topical Delivery of 5-Fluorouracil to Skin Cancer Cells: Ex-Vivo Release Study and Cytotoxicity Analysis
Harish SHARMA1,Gyanesh Kumar SAHU3,Swarnali Das PAUL3,Chanchal Deep KAUR1
1Department of Pharmaceutics, Shri Rawatpura Sarkar Institute of Pharmacy, Chhattisgarh-India
2Department of Pharmaceutics, Rungta Institute of Pharmaceutical Sciences, Chhattisgarh-India
3Department of Pharmaceutics, Faculty of Pharmaceutical Science, Shri Shankaracharya Group of Institute, Chhattisgarh-India
4Department of Pharmaceutics, Rungta College of Pharmaceutical Sciences and Research, Chhattisgarh-India
DOI : 10.5505/tjo.2022.3310 OBJECTIVE
The objective of the present work was to compare the efficacy of two formulations (microemulsion [ME] and nanoemulsion [NE]) with marketed product on skin cancer cell lines.

For this purpose, we studied two parameters, that is, drug release both in ex vivo conditions and cytotoxicity profile. For ex vivo skin permeability studies skin of male Sprague Dawley rats, goats, and human cadaver was selected. For in vitro cytotoxicity study, SK-MEL-5 melanoma cell lines were used. % cell death was concentration dependent.

The highest concentration (96 ?M) maximum cell death was found. The nanoemusion had highest inhibitory effect in any concentration than control or ME. Similar results were obtained for permeation study. NE had better permeation property than other.

Based on the permeation results, we found that highest amount of 5-fluorouracil collected in the viable part of the human cadaver and goat skin model from NE as compared to other preparations. Finally, it can be concluded that NE of the model drug had better properties in cytotoxicity and skin permeation than marketed topical product. Keywords : 5-Fluorouracil; microemusion; nanoemulsion; skin cancer